Polymerase chain reaction PCR is one of the most powerful and cheap laboratory methods. PCR appearance led to truly revolutionary changes in science and medicine. Perhaps the most valuable properties of this method are compatibility with other techniques and incredible flexibility, they allow biologists and doctors with minimal effort to solve completely different problems.
Polymerase chain reaction (PCR) is a molecular biology method that allows you to create copies of a specific DNA fragment from the original sample, increasing its content in the sample by several orders of magnitude.
In the process of PCR
Temperature Cycling (heating, cooling and thermal stabilization in time) of samples DNA in biological material is performed.
Among overs the use of PCR in clinical medicine:
- Analysis of clinical samples for the presence of numerous infectious agents of bacterial and viral nature as HIV, hepatitis and herpes viruses, chlamydia, Helicobacter pylori, tuberculous mycobacteria, including
Coronavirus Infection COVID-19
Diagnosis of the viruses is possible using real-time reverse transcription polymerase chain reaction.
The universality of the PCR method makes it indispensable and often the only one in a number of cases in such areas of medicine as:
- Infectious Medicine
- Diagnosis of Hereditary Diseases caused by mutations of individual genes: sickle cell anemia, amyotrophic lateral sclerosis, phenylketonuria, cystic fibrosis, muscle dystrophy, etc.
- Personalized Medicine
Not all medicines act equally on all people. The same substance can help one patient and be toxic or allergenic to another due to the characteristics of the metabolic processes of different people. Therefore, individually selected dosages of individually selected drugs are most effective. Metabolic differences are usually genetically determined. Having made the patient a kind of “genetic passport” of such features, it is possible to select the right treatment based on it.